Fig. 4: Antitumor response of p32 CAR T cells against gliomas in vivo.
From: P32-specific CAR T cells with dual antitumor and antiangiogenic therapeutic potential in gliomas
a Groups of 6–8 weeks C57BL/6J mice (N = 8) were transplanted with 3 × 105 005 GSC, and then adoptively transferred intravenously (IV) with either mCAR-SP6 (mSp6) or mCAR-p32 (mp32) T cells (1 × 107 total T cells) on days 10 and 17 post tumor implantation. b Groups of 6–8 weeks Nude mice (N = 6) were intracranially (IC) inoculated with 3 × 105 U87 cells, and then adoptively transferred with either human UT or CAR-p32 (hp32) T cells (1 × 107 total) on day 10 IC. c Groups of 6–8 weeks NUDE mice (N = 8) were IC inoculated with 3 × 104 GBM83 cells, and then adoptively transferred with either hUT or hp32 T cells (1 × 107 total) on day 3 IC and 2.5 × 106 total T cells intracerebroventricularly (ICV). Two-sided Mantel-Cox (log-rank) test was used to address differences in median survival in (a–c). d, e Representative bioluminescence images (d) and bioluminescence kinetics (e) of GBM83-luciferase-expressing cells engrafted in Nude mice. Data represent mean ± SEM, each dot represents one mouse, N = 8. Two-way ANOVA was used to analyze overall differences in both groups during the course of the experiment. f, g Analysis of p32 expression in GBM83 tumor-bearing mice following CAR T treatment. f Tumor sections (40 μm) were stained with p32 antibody and analyzed by confocal microscope (GFP = tumor area; DAPI = nuclei staining). Quantification was assessed by Fiji/ImageJ. Each dot shown in the graph represents average of three measurements done per slide. A total of eight slides originated in three different mice were stained per group. Unpaired t test was used. Two-tailed P value is shown. g, h Representative GBM83 tumors from each group were dissociated and analyzed by FACS for p32 surface expression on GFP+ tumor cells (g) and PD-1 expression of tumor infiltrated T lymphocytes (gated on CD3 + fraction) (h). Quantification of the results is summarized in the bars graph. Each dot represents the average value for one specific mouse (N = 3). Results are expressed as median ± SEM. For g, h unpaired t test was used, two-tailed P value is shown. All source data are available in the Source Data file.
