Fig. 8: TAAR enhancers function cooperatively.
From: Olfactory expression of trace amine-associated receptors requires cooperative cis-acting enhancers
a Generation of single T-element deletions via CRISPR. Locations of gRNAs (gray) are shown relative to TE1 and TE2 (magenta). The individual ΔTE1 and ΔTE2 mutations were generated on a chromosome harboring a previously targeted Taar1-YFP allele (green). Bottom, diagram of ΔTE1 and ΔTE2 in cis with Taar1-YFP. Deletion is marked (X). b, c Relative fold expression of TAAR (blue) and control OR (gray) genes measured using qPCR (2-ΔΔCt method) from olfactory mucosa from ΔTE1 and ΔTE2 mice. Error bars indicate mean ± SEM. Taar8 primers amplify all 3 family members. n = 5 mice per genotype. *p < 0.01; one-way ANOVA, Dunn–Šidák correction for multiple comparisons.
