Fig. 2: Bioavailability, biodistribution, and immunogenicity of a mouse surrogate Multabody.

a Binding kinetics of WT and Fc-modified (LALAP mutation) MB to mouse FcγRI (left) and mouse FcRn at endosomal (middle) and physiological (right) pH in comparison to the parental IgG. Two-fold dilution series from 100 to 3 nM (IgG) and 10 to 0.3 nM (MB) were used. Red lines represent raw data; black lines represent global fits. b Five male C57BL/6 mice per group were used to assess the serum concentration of a surrogate mouse MB, a Fc-modified MB (LALAP mutation), and parental mouse IgGs (IgG1 and IgG2a subtypes) after subcutaneous administration of 5 mg/kg. c MB and IgG2a samples were labeled with Alexa-647 for visualization of their biodistribution post subcutaneous injection into three male BALB/c mice/group via live noninvasive 2D whole body imaging. 15 nm fluorescently-labeled gold nanoparticles (GNP), which have a similar Rh value as the Multabody are shown as a comparator. d Five male C57BL/6 mice per group were used to assess any anti-drug-antibody response induced by the mouse surrogate Multabody in comparison to parental IgG and a species-mismatched malaria PfCSP peptide fused to Helicobacter pylori ferritin (HpFerr). Mean values ± SD of n =  5 mice is shown in (b) and (d). Source data of panels b and d are provided as a Source Data file.