Fig. 3: Protein engineering to multimerize IgG-like particles against SARS-CoV-2.
From: Multivalency transforms SARS-CoV-2 antibodies into ultrapotent neutralizers
a Schematic representation of the human apoferritin split design. b Negative stain electron micrograph of the MB. (Scale bar 50 nm, representative of two independent experiments). c Hydrodynamic radius (Rh) of the MB. d Avidity effect on the binding (apparent KD) of 4A8 (purple) and BD23 (gray) to the SARS-CoV-2 Spike. e Sensograms of BD23 IgG and MB with different Fc sequence variants binding to FcγRI (top row), FcRn at endosomal pH (middle row) and FcRn at physiological pH (bottom row). Red lines represent raw data whereas black lines represent global fits. f Neutralization of SARS-CoV-2 PsV by 4A8 and BD23 IgGs and MBs. Representative data of three biologically independent samples. The mean values ± SD for two technical replicates is shown in each neutralization plot. Median IC50 values of the three biologically independent replicates are indicated. Source data are provided as a Source Data file.
