Fig. 3: FFA and phospholipid responses to AFC.
From: Saturated free fatty acids and association with memory formation

a Hierarchical clustering heatmap, where each pixel represents the mean fold-change response (paired versus unpaired AFC) of the indicated analyte from 8 animals, in a given brain region. White pixels represent analytes whose change in abundance was not significant (two-tailed t-test p > 0.05). Dotted boxes highlight regions of the heatmap demonstrating strong activity-dependent change of a particular lipid class as described in the main text. The arrow shows the particularly strong response of myristic acid (C14:0). b Simplified heatmap indicating total response (paired versus unpaired AFC) of all lipids of a given class in each brain region. Dotted boxes correspond to those in a. c Multivariate analysis of the 24 FFA profiles representing 6 brain regions and 4 AFC conditions (Supplementary Fig. 4), where each dot represents 18 normalized mean FFA concentrations observed across 8 animals. The isomap algorithm was used for non-linear dimensionality reduction from 18 to 3 dimensions. The 3D projection has been manually rotated to highlight the differences between the profiles obtained for each AFC experimental condition. Dashed lines represent a manual assignment of the axes around which the profile datapoints were resolved, according to CPP treatment and AFC pairing. CeA—central amygdala, BLA—basolateral amygdala, PFC—prefrontal cortex, VH—ventral hippocampus, DH— dorsal hippocampus, CB—cerebellum, PA—phosphatidic acid, PC—phosphatidylcholine, PE—phosphatidylethanolamine, PG—phosphatidylglycerol, PS—phosphatidylserine. Source data are provided as a Source Data file.