Fig. 1: IP3R2 null mutant (IP3R2 KO) and Aldh1L1-CreER:IP3R2loxp/loxp (IP3R2 cKO) mice exhibit ASD-like behaviors.
From: Impaired calcium signaling in astrocytes modulates autism spectrum disorder-like behaviors in mice
a, b Impaired social interaction in IP3R2 KO mice in the three-chamber test. a Social preference (object versus stranger 1, S1-O, t14 = 2.353, P = 0.0338, n = 8). b Social novelty recognition (S2-S1, t14 = 1.341, P = 0.2013, n = 8). c Enhanced recognition memory in IP3R2 KO mice in the novel object recognition test (U = 20, P = 0.0232, n = 10). d, e Increased repetitive behavior in IP3R2 KO mice in the self-grooming test (d t18 = 2.471, P = 0.0251, n = 11/7) and the marble-burying test (e t14 = 2.488, P = 0.0261, n = 8). f, g Same as (a, b) but for IP3R2 cKO mice (f t19 = 2.873, P = 0.0097, n = 11/10; g t19 = 0.9303, P = 0.3639, n = 11/10). h Same as (c) but for IP3R2 cKO mice (U = 40, P = 0.7197, n = 9/10). i, j Same as (d, e) but for IP3R2 cKO mice (i t17 = 2.845, P = 0.0112, n = 10/9; j t19 = 5.633, P = 0.2 × 10−4, n = 11/10). k, l Same as (a, b) but for IP3R2 knockdown C57BL/6 J mice (k Control vs. ShRNA1, t16 = 0.3078, p = 0.0072, n = 8/10; Control vs. shRNA2, t16 = 0.3065, p = 0.0074, n = 8/10; l Control vs. shRNA1, t16 = 0.1498, p = 0.8828, n = 8/10; Control vs. shRNA2, t16 = 1.704, p = 0.1076, n = 8/10). m Same as (c) but for IP3R2 knockdown C57BL/6J mice. n, o Same as (d, e) but for IP3R2 knockdown C57BL/6J mice. WT, wild-type mice; KO, IP3R2 null mutant mice; control, Aldh1L1-CreER mice; cKO, IP3R2 cKO mice. Data are presented as mean ± SEM; two-tailed unpaired t test (a, b, d, e, f, g, i, j, k, l, m); Mann–Whitney U-test (c, h, n, o). *P < 0.05, **P < 0.01, ****P < 0.0001. Each data point represents an individual mouse. Comparisons with no asterisk had a P > 0.05 and were considered not significant. Source data are provided as a Source data file.
