Fig. 2: SARS-CoV-2 infection of adult deer mice.
From: SARS-CoV-2 infection and transmission in the North American deer mouse
a–m Eight to thirty two-week old female or male deer mice (P. maniculatus) were inoculated with 105 TCID50 or 106 TCID50 of SARS-CoV-2 by an intranasal route (i.n.) of administration and compared with age-matched uninfected controls. A summary table outlining the experimental design for the infection studies with reference to the corresponding figure panels is provided (Supplementary Table. 1). Solid lines indicate mean, error bars indicate SD. Dashed lines and dotted lines indicate the limit of detection for the TCID50 assay and qRT-PCR assay, respectively. a, b, Kaplan–Meier curve depicting survival data (a) and weight data (b) over the course of 21 days following SARS-CoV-2 exposure (challenge dose, 105 TCID50). c–f, Infectious viral load (filled in circles, left axis) and vRNA levels (empty circles, right axis) in the (c) nasal turbinates, (d) lung, (e) small intestine, and (f) colon (challenge dose, 105 TCID50). g, h, Infectious viral load (filled in circles with bars, left axis) and viral RNA levels (empty circles, right axis) in (g) oropharyngeal swabs and (h) rectal swab samples (challenge dose, 105 TCID50). i, Viral RNA levels in nasal washes at 6 dpi (infectious dose, 105 TCID50). The sample was obtained for 4/5 deer mice. j–l, Viral RNA levels in the (j) urine, (k) feces, and (l) blood at the indicated times postinfection (infectious dose, 106 TCID50). m, Viral RNA levels in the nasal turbinates, lung, and small intestines at 21 days postinfection (infectious dose, 105 TCID50). Data were collected from two independent experiments. (a, b, n = 6; c–f, n = 3/6, for uninfected/infected; g, h, n = 5/6; i–k, n = 3/4 (i) or 3/5 (j, k), for uninfected/infected; l, n = 5 (uninfected, 1 dpi) or 4 (3 dpi); m, n = 4 biologically independent samples (individual animals) were examined).
