Fig. 1: NETosis is inhibited in Kidneys of CFT073^wt induced pyelonephritis murine model and in CFT073^wt-treated neutrophils.

a Gross pathological observation of kidneys from CFT073^wt- or CFT073^Δtcpc- induced pyelonephritis mouse models. One representative kidney from five mice in each group was shown. n = 5. b Abscess formation of kidneys in mice with different treatment. c Histological examination of kidneys from CFT073^wt- or CFT073^Δtcpc-induced pyelonephritis mouse models. Scale bar = 25 μm. One representative image of kidneys from five mice in each group was shown, n = 3. d Confocal microscopy to examine in situ NETosis in kidneys from CFT073^wt- or CFT073^Δtcpc-induced pyelonephritis mouse models. Scale bar = 5 μm. e Percent and FI of co-localization reflecting the NETosis levels in experiments as described in d. Mean ± SD of data from five kidneys in each group were shown, n = 5. FI in the control group was set as 1.0, p < 0.05 and p < 0.01 were considered to be statistically significant and extremely significant respectively. NS not significant. f Examination of in vitro LPS-induced NETosis of CFT073^wt or CFT073^Δtcpc treated neutrophils by confocal microscopy. Scale bar = 5 μm. Images are representative of neutrophils from three different donors, n = 3. g Percent and FI of co-localization reflecting the NETosis levels in experiments as described in f. Mean ± SD of three independent experiments were shown, n = 3. p < 0.05 and p < 0.01 were considered to be statistically significant and extremely significant respectively. NS not significant. p-values were derived by Dunnett and Mann–Whitney tests. Source data for panel e, g are provided in the separate Source Data file.