Fig. 5: rTcpC inhibits protein but not mRNA levels of PAD4 in LPS-treated neutrophils. | Nature Communications

Fig. 5: rTcpC inhibits protein but not mRNA levels of PAD4 in LPS-treated neutrophils.

From: TcpC inhibits neutrophil extracellular trap formation by enhancing ubiquitination mediated degradation of peptidylarginine deiminase 4

Fig. 5

a qRT-PCR to examine mRNA levels of PAD4 in different treatment groups of neutrophils. Data are from three independent experiments (n = 3) with three replicates per condition. b Western blot analyses of PAD4 protein levels in neutrophils with different treatment. c Gray scale analyses of bands from experiments as described in b. Gray scale values reflecting the PAD4 levels in the control group were set as 1.0. Mean ± SD of three independent experiments were shown. p < 0.05 and p < 0.01 were considered to be statistically significant and extremely significant respectively, NS not significant. d Dynamic analyses of the influence of rTcpC on pad4 mRNA levels in neutrophils with different treatment. The data of pad4 mRNA levels are from 3 independent experiments (n = 3). e Dynamic analyses of PAD4 in neutrophils with different treatment by western blot. f Gray scale analyses of bands from experiment as described in e. Mean ± SD of three independent experiments were shown. p-values were derived by Dunnett and Mann–Whitney tests. All western blots in panels b, e are provided as uncropped blots in Gels and Blots of Source Data file. Source data for panel a, c, d, f are provided in the separate Source Data file.

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