Fig. 7: rTcpC treatment leads to accumulation of PAD4 in proteasomes. | Nature Communications

Fig. 7: rTcpC treatment leads to accumulation of PAD4 in proteasomes.

From: TcpC inhibits neutrophil extracellular trap formation by enhancing ubiquitination mediated degradation of peptidylarginine deiminase 4

Fig. 7

a Dynamic observation of co-localization of PAD4 with PSMD2 by confocal microscopy. Scale bar = 5 μm. b Enlarged image of the co-localization in a at 210 min. Scale bar = 2 μm. Confocal microscopy images are representative of three independent experiments from three donors’ neutrophils, n = 3. c Percent and FI of co-localization were analyzed by ImageJ software. Mean ± SD of three independent experiments as described in a were shown. p < 0.05 and p < 0.01 compared with the group at 0 min. d Dynamic observation of co-localization of PAD4 with PSMD2 in the presence of MG-132 by confocal microscopy. Scale bar = 5 μm. e Enlarged image of the co-localization in d at 210 min. Scale bar = 2 μm. Confocal microscopy images are representative of three independent experiments from three donors’ neutrophils, n = 3. f Percent and FI of co-localization were analyzed by ImageJ software. Mean ± SD of three independent experiments as described in d were shown. p < 0.05 and p < 0.01 compared with the group at 0 min. p-values were derived by Dunnett test. Source data for panel c and f are provided in the separate Source Data file.

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