Fig. 8: rTcpC-C12S loses ability to promote degradation of PAD4 and inhibit LPS-induced NETosis. | Nature Communications

Fig. 8: rTcpC-C12S loses ability to promote degradation of PAD4 and inhibit LPS-induced NETosis.

From: TcpC inhibits neutrophil extracellular trap formation by enhancing ubiquitination mediated degradation of peptidylarginine deiminase 4

Fig. 8

a Western blot analyses to detect the ability of rTcpC-C12S to promote rPAD4 degradation. One representative of three independent experiments was shown, n = 3. b Gray scale analyses of bands from experiments as described in a. Gray scale values reflecting the PAD4 levels in the control group were set as 1.0. Mean ± SD of three independent experiments were shown. p < 0.05 and p < 0.01 were considered to be statistically significant and extremely significant respectively, NS not significant. c Confocal microscopy to detect the ability of rTcpC-C12S to inhibit LPS-induced NETosis. Scale bar = 5 μm. d Percent of co-localization was analyzed by ImageJ software. Mean ± SD of three independent experiments as described in c, p < 0.01 was considered to extremely significant. e FI of co-localization was analyzed by ImageJ software. FI in control group was set as 1.0. Mean ± SD of three independent experiments as described in c were shown, n = 3, p < 0.01 was extremely significant. All western blots in panels a are provided as uncropped blots in Gels and Blots of Source Data file. p-values were derived by Dunnett and Mann–Whitney multiple comparison tests. Source data for panel b, d and e are provided in the separate Source Data file.

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