Fig. 6: Time-dependent proliferation of TH+ cells in CRISPR/Cas9 MPS-IIIA cell line. | Nature Communications

Fig. 6: Time-dependent proliferation of TH+ cells in CRISPR/Cas9 MPS-IIIA cell line.

From: Altered heparan sulfate metabolism during development triggers dopamine-dependent autistic-behaviours in models of lysosomal storage disorders

Fig. 6

a SH-SY5Y MPS-IIIA showed increased cell density at DIV4, compared to WT controls, but not at DIV1. Representative staining for each condition are reported (scale bar: 10 µm). b Similarly, while at DIV1 there was no difference in the DA release, at DIV4 MPS-IIIA cells increased DA release. c MTS, and d BrdU assays confirmed that SH-SY5Y MPS-IIIA cells show an increased proliferation rate, compared to WT cells at DIV4 but not at DIV1. Histograms represent mean ± S.E.M. §p < 0.05 vs DIV1, within groups; *p < 0.05 vs WT, between groups.

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