Fig. 7: Replacement of functional heparan sulfate partially rescued the increased proliferation in the SH-SY5Y MPS-IIIA cell line. | Nature Communications

Fig. 7: Replacement of functional heparan sulfate partially rescued the increased proliferation in the SH-SY5Y MPS-IIIA cell line.

From: Altered heparan sulfate metabolism during development triggers dopamine-dependent autistic-behaviours in models of lysosomal storage disorders

Fig. 7

a, aʹ CRISPR/Cas9 MPS-IIIA transfected with a plasmid expressing hSGSH gene with a myc-FLAG tag showed a decreased percentage of TH+ cells, compared to non-transfected cells. aʺ, a‴ Similarly, MTS and BrdU assay showed a significant decrease in MPS-IIIA transfected cells. b, bʹ HS treatment (25 or 40 µg/mL) decreased the number of TH+ cells, and bʺ, b‴ the proliferation rate, evidenced with MTS and BrdU assays. Representative staining for each condition is reported (scale bar: 10 µm). Data are expressed as mean ± S.E.M. *p < 0.05, between groups; #p < 0.05 vs MPS-IIIA vehicle, within group.

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