Fig. 2: LINC00842 alters metabolic programs in PDAC cells. | Nature Communications

Fig. 2: LINC00842 alters metabolic programs in PDAC cells.

From: LINC00842 inactivates transcription co-regulator PGC-1α to promote pancreatic cancer malignancy through metabolic remodelling

Fig. 2

a Differentially expressed genes identified by RNA sequencing in PDAC cells with or without LINC00842 knockdown (KD). Upregulated genes (fold change ≥2) and downregulated genes (fold change ≤0.5) were selected for pathway enrichment analysis. b Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of differentially expressed genes regulated by LINC00842. The dotted line indicates P < 0.05 (two-tailed). c Validation of some RNA-sequencing results by using quantitative RT-PCR. Data were normalized to control or KD-control. TCA, tricarboxylic acid cycle; OXPHOS, oxidative phosphorylation. d, e Effects of LINC00842 overexpression (LINC-OE) or silence (LINC-KD) on oxygen consumption rate (OCR) (d) or extracellular acidification rate (ECAR) (e). f Effects of LINC00842 expression change on glucose uptake or lactate production. g, h Representative pictures of Nile red (red) and DAPI (blue) staining of cells with LINC00842 overexpression or silencing (g) and quantification using Image J software (h). Scale bar, 30 μm. Results in (c), (f), and (h) are mean ± SD from 3 independent experiments. The P value in (f), (h) and *P < 0.05; **P < 0.01, and ***P < 0.001 in (c) were determined by Student’s t-test (two-tailed).

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