Fig. 1: Experimental workflow.

Freshly resected glioma specimens were acquired from patients representing major glioma malignancy groups: pilocytic astrocytomas (WHO grade I, PA, n = 11), diffuse astrocytomas (WHO grade II and III, DA, n = 7) and glioblastomas (WHO grade IV, GBM, n = 15). Clinical data about each patient was collected. Glioma tissues were homogenized to a single cell suspension and experimentally defined numbers of cells were split into portions that were processed for RNA-seq, ATAC-seq, ChIP-seq, or DNA methylation experiments. Differential analysis of epigenomic and transciptomic profiles across glioma grades was performed, followed by identification of related regulatory mechanisms. Results from all genome-wide experiments were made available in a genome browser (http://regulomics.mimuw.edu.pl/GliomaAtlas/).