Fig. 9: Short term Rapamycin treatment rescues the premature increase of PV cell perisomatic innervation caused by Tsc1 haploinsufficiency. | Nature Communications

Fig. 9: Short term Rapamycin treatment rescues the premature increase of PV cell perisomatic innervation caused by Tsc1 haploinsufficiency.

From: Sensitive period for rescuing parvalbumin interneurons connectivity and social behavior deficits caused by TSC1 loss

Fig. 9

a Schematic of the treatment paradigm. b–m Representative immunostained sections of somatosensory cortex labeled for PV/GFP (blue/green) and gephyrin (red) in Vehicle (b–g) and Rapamycin (i–m) treated P22 mice showing PV/GFP-Gephyrin colocalized boutons (arrowheads) (d, g and j, m). Scale bar: 5 μm. n, o PV/GFP-Gephyrin colocalized puncta in Vehicle-treated (n, Welch’s t-test, *p = 0.0423) and Rapamycin-treated mice (o, Welch’s t-test, p = 0.6088). Vehicle treatment: n = 5 for both PV-Cre;RCE;Tsc1+/+ and PV-Cre;RCE;Tsc1flox/+ mice. Rapamycin treatment: n = 5 PV-Cre;RCE;Tsc1+/+, n = 6 PV-Cre;RCE;Tsc1flox/+ mice. Data represent mean ± SEM. Source data are provided as a Source Data file.

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