Fig. 7: Differential gene expression analysis in N-87 cancer cells after anti-HER2 treatments by RNA-seq reveals distinct gene up- and downregulation caused by 441.
From: Engineering an anti-HER2 biparatopic antibody with a multimodal mechanism of action
a MDS plot for indicated treatment in biological replicates (A/B). b Limma/voom fit of log2 transformed counts per million reads (CPM) and calculation of quality weights for individual samples. c MDplot of significant DEG’s versus PBS control treatment. d Venn diagram analysis of significant DEG’s with twofold change (log2FC > 1). e Euclidean clustered heat map based on z-scores of significantly regulated transcripts after the 441 treatment across all indicated treatments. f Euclidean clustered heat map of hallmark gene sets after indicated treatments. Note, adjusted p values were −log10-transformed and downregulated gene sets were multiplied by −1 to account for negative effect. Source data are available in the Source Data file.
