Fig. 1: Schematic illustration of a platform for high-throughput screening and rational design of the biofunctionalized surfaces with optimized biocompatibility and antimicrobial activity. | Nature Communications

Fig. 1: Schematic illustration of a platform for high-throughput screening and rational design of the biofunctionalized surfaces with optimized biocompatibility and antimicrobial activity.

From: High-throughput screening and rational design of biofunctionalized surfaces with optimized biocompatibility and antimicrobial activity

Fig. 1

a Ti substrates were treated with sodium hydroxide to realize hydroxylation. b Hydroxylated Ti substrates were reacted with silane-PEG2000-MAL solution to introduce alkene bonds. c By the thiol–ene click chemistry, utilizing a combination of “evaporation” and “immersion” techniques to construct AMP and RGD dual-functionalized gradient surfaces. d Studying the biological properties of mBMSCs and S. aureus on the AMP and RGD dual-functionalized gradient surfaces, to identify the best region which can promote both cell adhesion and bacteria killing and thus to obtain the best parameters for producing the optimized AMP and RGD dual-functionalized surfaces. e The optimized AMP and RGD dual-functionalized surfaces were designed by the extracted parameters to achieve excellent in vitro and in vivo biocompatibility and antimicrobial activity for successful BAI inhibition and bone defect repair.

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