Fig. 1: Incorporation of deuterium into palmitate and detection by MS.
From: Measurement of lipogenic flux by deuterium resolved mass spectrometry
a 2H is incorporated into acetyl-CoA during nutrient metabolism and subsequently into palmitate during the reactions of FASn. A newly synthesized palmitate will contain 2H from body water, NADPH, malonyl-CoA, and acetyl-CoA. The isotopic enrichment of palmitate measured by GC–MS can be used to quantify the fraction of newly synthesized palmitate. b Simulation of unit resolution mass spectra of palmitate in the setting of 0.3% 2H body-water enrichment and fractional DNL fluxes of 0–100%. c Simulations of the high-resolution mass spectra of palmitate under the same conditions. Simulations are available as Source Data.
