Fig. 4: Detection of DNL by high resolution MS is responsive to physiology in mice and humans.
From: Measurement of lipogenic flux by deuterium resolved mass spectrometry
a Detection of nocturnal feeding in mice at the various doses of 2H2O (20, 5, 2.5, or 1 μL/g body weight). Colors correspond to the doses as in Fig. 3. DNL in fasted/refed mice was measured in the daytime following a fasting/refeeding protocol (between 9 am and 1 pm) and again in the same mice the next morning. Each point represents an individual mouse administered the indicated 2H2O dose (n = 19 mice). b DNL measured by either high resolution detection of 2H palmitate or 3H radioactivity in hepatic triglycerides of SREBP-1a transgenic mice 1-h after a 500 μl injection of 99.9% 2H2O (≈20 µl/g) or 50 mCi of 3H2O (n = 3 mice per group). c Evolution of deuterium enrichment in plasma TG palmitate after an overnight fast and a ~1 g/Kg body-weight dose of water (n = 4 human subjects). d Fractional DNL (%) over time following a 2H2O dose at 0-h and a liquid meal at 2-h (n = 4 human subjects). Significance was determined by a two-sided paired (a) or unpaired t-test (b). Mean and SEM are shown where indicated.
