Fig. 3: HiBiT fused to EEP-modified GFP retains luminescent activity. | Nature Communications

Fig. 3: HiBiT fused to EEP-modified GFP retains luminescent activity.

From: Unravelling cytosolic delivery of cell penetrating peptides with a quantitative endosomal escape assay

Fig. 3

a SDS-PAGE gel of EEP-GFP-HiBiT fusion proteins. n = 3 independent experiments b Luminescent activity of EEP-GFP-HiBiT relative to HiBiT peptide (activity of HiBiT normalised to 1). For simplicity in naming, EEP-GFP-HiBiT fusion proteins are labelled by the EEP. Data represents mean ± SEM, n = 3 independent experiments. Two-tailed unpaired t test was used to analyse the data. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 and ****p ≤ 0.0001. c Summary of EEP peptide sequences, their respective charges and the overall charge when fused to GFP-HiBiT. EEPs are listed in order of decreasing charge. Source data are provided as a Source Data file.

Back to article page