Fig. 6: EEPs do not increase endosomal escape efficiency of GFP.

a Cytosolic luminescent signal of EEP-GFP-HiBiT in HEK293-LSA cells and fold-increase in signal with respect to GFP (represented by dotted line = 1). HEK293-LSA cells were incubated with EEP-GFP-HiBiT proteins at varying concentrations (refer to methods) for 4 h. Data represents mean ± SEM, n = 3 independent experiments. Kruskal–Wallis with uncorrected Dunn’s test was used to analyse the data. b Total cellular association of EEP-GFP-HiBiT in HEK293 cells and fold-increase with respect to GFP-HiBiT (represented by dotted line = 1) determined by permeabilising the cells using 0.01% w/v digitonin. Data represents mean ± SEM, n = 3 independent experiments. Kruskal–Wallis with uncorrected Dunn’s test was used to analyse the data. c Endosomal escape efficiency of EEP-GFP-HiBiT proteins determined by ratioing cytosolic signal with total cellular association. Data represents mean ± SEM, n = 3 independent experiments. Kruskal–Wallis with uncorrected Dunn’s test was used to analyse the data. d Summary of cytosolic luminescence, total cellular association luminescence and endosomal escape efficiency for all proteins. Data represents mean ± SEM, n = 3 independent experiments. Kruskal–Wallis with uncorrected Dunn’s test was used to analyse the data, with GFP as the control group. Data represents mean ± SEM, n = 3 independent experiments. ns (not significant) denotes p > 0.05, *p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001. Source data are provided as a Source Data file.