Fig. 1: scRNA-seq suggests inverse correlation between TAM and CD8 T cells. | Nature Communications

Fig. 1: scRNA-seq suggests inverse correlation between TAM and CD8 T cells.

From: Single-cell RNA sequencing shows the immunosuppressive landscape and tumor heterogeneity of HBV-associated hepatocellular carcinoma

Fig. 1

a Workflow of the experiment. b Stratification and cell-type identification of HCC single cells. Malignant cells (labeled by the case identity) and non-malignant cells (labeled by cell types) were grouped into distinctive cell clusters. c Single cells from the 8 HCC cases were stratified into 34 cell clusters using a graph-based Louvain clustering algorithm on the KNN graph (resolution = 1.2). d Summary of different major cell types identified in HCC tumors. e Proportion of tumor-infiltrating macrophages was inversely correlated to the proportion of tumor-infiltrating T cells in scRNA-seq and deconvoluted bulk-cell RNA-seq datasets. Pearson correlation (two-sided). f Immunohistochemistry of CD8 T cells and CD163 M2 macrophages in two representative HCC cases of an independent cohort. Scale bar = 100 µm. More than ten fields each under ×40 and ×100 magnification were examined. Source data are provided as a Source Data file.

Back to article page