Fig. 5: Effects of SAP on myelomonocytic cell function.
From: Serum amyloid P component is an essential element of resistance against Aspergillus fumigatus
a FACS analysis of phagocytosis (1 and 20 min) of FITC-labeled AF conidia opsonized with murine SAP by peripheral murine neutrophils (Ly6G+ CD11b+), monocytes (Ly6C+ CD11b+) and DCs (CD11c+) (1 × 107 conidia/200 µl of blood). N = 7 wt, n = 8 Apcs−/−. wt vs. wt + Sap, ****P < 0.0001 (1 min), ***P = 0.003 (20 min) (neutrophils); ****P < 0.0001 (1 min), ***P = 0.002 (20 min) (monocytes). Apcs−/− vs. Apcs−/− + Sap, ***P = 0.0004 (1 min), ****P < 0.0001 (20 min) (neutrophils); **P = 0.005 (1 min), ****P < 0.0001 (20 min) (monocytes) (two-sided, unpaired t-test). b Effect of human SAP opsonisation on in vitro phagocytosis by isolated human monocytes (n = 4 donors) (1 × 105 cells and 1 × 106 FITC-labeled AF conidia; 30 min; 10% of autologous serum). *P = 0.03 (two-sided, unpaired t-test). c Levels of CXCL1, CXCL2, TNF-α, and IL-6 in supernatants of bone marrow-derived macrophages from wt and Apcs−/− mice stimulated (24 h) with A. fumigatus conidia (ratio 1:5, cell:conidia) in the presence of correspondent mouse serum (10%). LPS, 10 ng/ml. n = 4 mice per genotype. d FACS analysis of FITC-labeled AF conidia phagocytosis by neutrophils (CD45+ Ly6G+), macrophages (CD45+ Ly6C+ F4/80+) and DCs (CD45+ CD11c+) collected from BALFs of wt (−, n = 7; Sap, n = 5) and Apcs−/− (−, n = 8; Sap, n = 5) mice after i.t. injection of 5 × 107 conidia (4 h) opsonized with murine SAP. d Neutrophils, wt vs. Apcs−/−, *, P = 0.05; wt vs. wt + Sap, *, P = 0.03; ***, P = 0.0003. Macrophages, *P = 0.030; ***P = 0.004 (two-sided, unpaired t-test). a–d Each spot corresponds to a single mouse (a, c, d) or single healthy donor (b). a–d Mean ± SEM of independent experiments.
