Fig. 5: Expression of FYN-TRAF3IP2 in hematopoietic cells fosters the development of PTCL-NOS. | Nature Communications

Fig. 5: Expression of FYN-TRAF3IP2 in hematopoietic cells fosters the development of PTCL-NOS.

From: Fusion transcripts FYN-TRAF3IP2 and KHDRBS1-LCK hijack T cell receptor signaling in peripheral T-cell lymphoma, not otherwise specified

Fig. 5

a Kaplan–Meier survival curve after transplantation with HSPC transduced with empty pMIG vector (n = 5), pMIG-FYN-TRAF3IP2 (n = 10) or pMIG-FYNG2A-TRAF3IP2-GFP (n = 6). Log-rank p value was obtained from a two-sided Chi-square test. b Spleen weights at sacrifice of mice transplanted with HSPC transduced with empty vector (EV, n = 5), FYN-TRAF3IP2 (FT, n = 9) or FYNG2A-TRAF3IP2 (FG2AT, n = 6). p values were calculated with Tukey’s post-hoc multiple comparisons test. c Representative H&E stains of lymph nodes from mice transplanted with HSPC transduced with empty vector (n = 5), FYN-TRAF3IP2 (n = 5) or FYNG2A-TRAF3IP2 (n = 5). Low magnification images are in the top row, scalebars represent 500 µm. High magnification images are in the bottom row, scalebars represent 50 µm. d Representative flow cytometry plots for cell suspensions from the lymph nodes of EV mice, FT mice and FG2AT mice (top). Quantification of the lineage of GFP+ cells in lymph node cell suspensions (bottom). n = 5 mice per group. p values were calculated with Tukey’s post-hoc multiple comparisons test. e Representative H&E stains of livers from mice transplanted with HSPC transduced with empty vector (n = 5), FYN-TRAF3IP2 (n = 5) or FYNG2A-TRAF3IP2 (n = 5). Low magnification images are in the top row, scalebars represent 500 µm. High magnification images are in the bottom row, scalebars represent 50 µm. f Representative picture of GFP immunohistochemistry on liver sections of mice with FYN-TRAF3IP2-expressing cells (n = 5). Scalebar represents 200 µm. g Western blot analysis for TRAF3IP2 and GFP on lymph node lysates after transplantation with HSPC transduced with empty vector, pMIG-FYN-TRAF3IP2 or pMIG-FYNG2A-TRAF3IP2. h Representative gel analysis pictures for RT-PCR analysis of Tcrb rearrangements (left to right: Trbv6, Trbv1, Trbv26, Trbv2, Trbv12-1/12-2/12-3, Trbv19, Trbv29, Trbv13-1/13-2/13-3, Trbv17, Trbv4, Trbv16, Trbv15, Trbv14, Trbv31, Trbv20, Trbv3, Trbv18, Trbv30 and Trbv21) and Ciz control reaction (far right lane) on lymph nodes from an empty vector control mouse (top, n = 2) and a FYN-TRAF3IP2-induced lymphoma (bottom, n = 5). All data are represented as mean ± SD.

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