Fig. 7: FYN-TRAF3IP2 activates canonical NF-κB signaling in vivo.

a Volcano plots of differentially expressed genes in CD4⁺GFP⁺ lymphoma cells versus naive CD4⁺ T cells (left), CD4⁺GFP⁻ stromal T cells (middle) and CD4⁺GFP⁺ FYN^G2A-TRAF3IP2-expressing T cells (right). Genes significantly upregulated (²logFoldChange > 1 and ‒¹⁰logp_adj > 2) in lymphoma cells are maroon-colored, genes significantly downregulated (²logFoldChange < −1 and −¹⁰logp_adj > 2) in lymphoma cells are depicted in blue. Motifs from cis-regulatory features associated with differentially expressed genes are depicted. b Enrichment plots for a list of manually curated NF-κB target genes (top row), p65 target genes identified by ChIP-seq (middle row) and computationally predicted p65 target genes (bottom row) in lymphoma cells compared with naive CD4⁺ T cells (left column), CD4⁺GFP⁻ stromal T cells (middle column) and CD4⁺GFP⁺ FYN^G2A-TRAF3IP2-expressing T cells (right column). c Quantification of cells with nuclear p65 positivity as a fraction of all cells in the lymphoma or as a fraction of GFP⁺ lymphoma cells in FYN-TRAF3IP2-induced lymphomas (top) and representative images (bottom). n = 5 mice. p value is derived from the cumulative distribution function of a hypergeometric distribution. Scalebars represent 10 µm.