Fig. 4: Enhancing butyl acetate production through rational organization of the enzymes associated with BA synthesis. | Nature Communications

Fig. 4: Enhancing butyl acetate production through rational organization of the enzymes associated with BA synthesis.

From: Renewable fatty acid ester production in Clostridium

Fig. 4

a Biological components evaluated in this study for rational organization of enzymes: CC-Di-A and CC-Di-B tags, PduA* scaffold, and MinD C-tag. b Schematic representation of assembling two of the three enzymes for BA synthesis with the CC-Di-A and CC-Di-B tags. c Schematic representation of organizing the three enzymes for BA synthesis onto the PduA* formed scaffold. d Schematic representation of using the MinD C-tag to draw ATF1 onto the cell membrane. e The fermentation results for butyl acetate (BA) production with various mutant strains corresponding to the genetic manipulations from (b–d). Data are presented as mean values ± SD (n = 3). Source data underlying Fig. 4e are provided as a Source Data file.

Back to article page