Fig. 5: The deletion of the prophage genomes.

a The gene cluster organization of the TBP2 prophage (P1) and other four putative prophages (P2-P5). b Cell growth profiles of ∆P1234 with the induction (at various OD₆₀₀) using mitomycin C at 2 or 4 µg/mL. The value at the right side of the cell growth profile figure represents the actual OD₆₀₀ value at which mitomycin C (with the applied concentration included in the parentheses) was added for the induction. Data are presented as mean values ± SD (n = 3). c Transmission electron microscopy image of Clostocin O (observed in the supernatant of ∆P1234 after induction with mitomycin C). Similar results were observed in six independent experiments. d Cell growth profiles of ∆P12345 with the induction (at various OD₆₀₀) using mitomycin C at 2 or 4 µg/mL. The value at the right side of the cell growth profile figure represents the actual OD₆₀₀ value at which mitomycin C (with the applied concentration included in the parentheses) was added for the induction. Data are presented as mean values ± SD (n = 3). e Transmission electron microscopy image of the phage TBP2 particles (observed in the supernatant of ∆P2345 after induction with mitomycin C). Similar results were observed in two independent experiments. f Comparison of the cell growth of prophage deleted mutants and the control N1-4-C strain. Data are presented as mean values ± SD (n = 3). g Comparison of the butanol production in prophage deleted mutants and the control N1-4-C strain. Data are presented as mean values ± SD (n = 3). h Comparison of the cell growth between ∆P1234 and ∆P12345. Data are presented as mean values ± SD (n = 3). i Comparison of butanol production between ∆P1234 and ∆P12345. Data are presented as mean values ± SD (n = 3). Source data underlying Fig. 5b–i are provided as a Source Data file.