Fig. 1: Construct design of C9orf72:ARF1-SMCR8:WDR41 complex.
From: Structural basis for the ARF GAP activity and specificity of the C9orf72 complex
a Diagram of the complex design. ARF1 is fused to the N-terminus of SMCR8 subunit. The blue star represents the position of the catalytic Arg residue of SMCR8. b Left, the Superose 6 size exclusion profile of the reconstituted ARF1-SMCR8:C9orf72:WDR41 complex. Right, the SDS-PAGE analysis of the peak fractions for ARF1-SMCR8:C9orf72:WDR41 complex compared with wild-type complex. c The HPLC analysis of the purified C9orf72:SMCR8:WDR41, C9orf72:ARF1-SMCR8:WDR41 complex, and ARF1. The elution profile for GDP/GTP is shown on the top panel.
