Fig. 4: Interfaces between ARF1 and C9orf72/SMCR8 and HPLC result of ARF1WT/mutants with C9orf72WT/mutants:SMCR8:WDR41.
From: Structural basis for the ARF GAP activity and specificity of the C9orf72 complex
a Close-up views of the interfaces between switch I/switch II regions of ARF1 with C9orf72:SMCR8. b HPLC analysis of ARF1WT/mutants incubated with C9orf72:SMCR8:WDR41. The top panels are the control reactions for each ARF1 proteins, either wild-type or mutants. The bottom ones are incubated with 1/100 molar ratio of C9orf72:SMCR8:WDR41. c ARF1 or RAB8A incubated with 1/100 or 1/10 molar ratio of C9orf72WT/mutants:SMCR8:WDR41, respectively. The experiments in b, c are carried out at least three times, and one representative plot is shown.
