Fig. 2: PER2- and CRY1-fusion protein oscillate in single knock-in cells.

a, c Montage of fluorescence microscopy images of selected individual U-2 OS single knock-in cells’ nuclei over the course of 3 days after synchronization. b, d Mean of nuclear fluorescence intensity (background-subtracted) quantified from (a) and (c). Cell division marked by (x). e Time series of normalized mean nuclear fluorescence in individual knock-in cells with average signal overlaid in bold. f Percentage of significantly rhythmic time series from (e). g–i Extracted rhythm parameters of significantly rhythmic single-cell time series from e (n = individual cells as stated in f). p-values: one-way ANOVA, two-sided. Scale bar: 10 µm. Boxplots: box: interquartile range, center: median, whiskers: minimum to maximum, mean is marked with (+). In b, d and e, the first 24 hours after synchronization (possible acute response to dexamethasone) are highlighted by a gray box. Source data are provided as a Source Data file.