Fig. 3: Simultaneous visualization of PER2- and CRY1-fusion protein oscillations in double knock-in cells.
From: Live-cell imaging of circadian clock protein dynamics in CRISPR-generated knock-in cells
a PER2- and CRY1-fusion protein oscillation in individual double knock-in cells. Fluorescence images of selected double-knock in clones at different times after synchronization. b Montage of bicolor fluorescence microscopy images of an individual U-2 OS double-knock-in cell’s nucleus over the course of 3 days after synchronization. c Mean nuclear fluorescence intensity (background-subtracted) quantified from (b). Cell division marked by (x). d Time series of normalized mean nuclear fluorescence in individual double knock-in cells. e Percentage of significantly rhythmic time series from d (n = individual cells as stated in f). f–h Extracted rhythm parameters of significantly rhythmic single-cell time series from d. p-values: Mann-Whitney-U test, two-sided, for h, p-value = 2*10−15. Boxplots: box: interquartile range, center: median, whiskers: minimum to maximum, mean is marked by (+). Scale bar: 10 µm. The first 24 hours after synchronization (possible acute response to dexamethasone) are highlighted by a gray box (c) or bar (d). ZT = zeitgeber time. Source data are provided as a Source Data file.
