Fig. 4: Increased immune cell infiltration in kidneys of mice 72 h after systemic infection with t-EED1+ yeast coincides with increased local cytokine production while systemic inflammation is not affected.

Mice were systemically infected with an intermediate dose of 2.5 × 10⁴ CFU/g body weight of WT (THE1-CIp10), t-EED1 or remained uninfected in the presence (+) of doxycycline. a Immune cells infiltrating the kidney 72 h post infection. Shown are absolute numbers of living immune cells per kidney. Two independent experiments, n = 10. b Cytokine levels were measured in kidney homogenates 24 h and 72 h post infection. Asterisks above bars represent significant differences compared to the uninfected control. Two independent experiments, n = 10, except for uninfected+, n = 9. MIP-1α, TNF-α for uninfected+, n = 5. IL-10 data derived from one experiment, n = 5. c Markers for systemic inflammation sTREM-1 and NGAL were quantified by ELISA in serum of mice after 6, 24, 48, 72 h and when mice become moribund (mor). Dashed lines indicate median serum protein levels of uninfected controls in the presence (dark gray) or absence of doxycycline (light gray). Two independent experiments. sTREM-1 n = 10, except for 6 h n = 6 and mor n = 5; for WT- 24 h n = 8 and 48 h n = 9. NGAL n = 10, except for WT− 24 h n = 9, t-EED1− 24 h n = 8 and 72 h n = 9. a–c Shown is the median and interquartile range, two-sided Mann–Whitney test. a, b p-values are shown in the graph and b asterisks indicate significant differences in comparison to the uninfected control (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001). Source data are provided as a Source Data file.