Fig. 5: TERRA transcription inhibition leads to accumulation of TFEs. | Nature Communications

Fig. 5: TERRA transcription inhibition leads to accumulation of TFEs.

From: TERRA transcription destabilizes telomere integrity to initiate break-induced replication in human ALT cells

Fig. 5

a Examples of telomeric DNA FISH on metaphases from the indicated cell lines. For TERRA transcription inhibition, nls3, sid1, and sid4 cells were treated with dox for up to 15 days (d). For POLD3 depletion, T-REx-U2OS cells were transfected twice three days apart with two independent siRNAs against POLD3 (siPD3a and siPD3c) or with control siRNAs (siCt) and harvested 6 days from the first transfection. Telomeric repeat DNA is in red, DAPI-stained chromosomal DNA in blue. White arrowheads point to chromosome arms with TFEs. Scale bar: 10 μm. b Quantifications of experiments as in a. Boxplots represent the medians (middle lines) and the first and third quartiles (boxes), the whiskers extend to the 90th percentile of the values. A total of at least 2761 chromosomes from 2 or 3 independent experiments were analyzed for each condition. P-values were calculated with a two-tailed Mann–Whitney U test. P1 = 0.0092; P2 = 0.0109; P3 = < 0.0001; P4 < 0.0001; P5 < 0.0001; P6 < 0.0001; P7 < 0.0001. Source data are provided as a Source Data file.

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