Fig. 6: TERRA transcription inhibition leads to transcriptomic changes. | Nature Communications

Fig. 6: TERRA transcription inhibition leads to transcriptomic changes.

From: TERRA transcription destabilizes telomere integrity to initiate break-induced replication in human ALT cells

Fig. 6

a Volcano plot of RNA-seq transcriptome data displaying the pattern of gene expression values in the comparison of sid4 cells treated with dox for 72 h versus untreated sid4 cells. Significantly differentially expressed genes (P ≤ 0.05 of Benjamini–Hochberg correction of nominal P-values obtained from the applied two-sided Wald test) are in red. Genes involved in DNA metabolism are indicated. b Bar plot representation of the Gene Ontology analysis of differentially expressed genes identifying significantly enriched biological process functional categories (FDR P ≤ 0.05). Statistical significance for each term is indicated on the X axis by the −log10 of the FDR P-value. c Western blot analysis of DNA metabolism factors identified in a. The indicated cell lines were treated with dox for 24 or 72 h or left untreated. Images are from different identical membranes. PCNA, Beta Actin (ACTB) and Lamin B1 (LMNB1) serve as loading controls. For HROB, the two asterisks indicate variants 1 and 4, the single asterisk variant 3. Images are representative of experiments repeated at least twice. Source data are provided as a Source Data file.

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