Fig. 2: Main properties of ATP-K72 coacervate droplets.
From: Active coacervate droplets are protocells that grow and resist Ostwald ripening
a ATP-K72 droplets containing Alexa Fluor-647-labeled pyruvate kinase. Channels are shown separately: gray (left)—transmission, green (middle)—GFP (attached to K72), magenta (right)—Alexa Fluor-647. K72 always contains the GFP tag; PyK was labeled with Alexa-647 only for this experiment. Scale bar: 10 μm. b The phase diagrams of ADP-K72 and ATP-K72 mixtures confirm that the conversion of ADP to ATP can induce coacervation under certain conditions and lead to growth (e.g., along the red line). The dashed lines representing the approximate phase boundaries are meant as a guide to the eye. Error bars represent one standard deviation of the intercept value in the linear fit used to determine critical salt concentration from a turbidity assay (see “Methods”, based on five turbidity measurements). Source data are provided in Supplementary Data 1. c Partitioning coefficients of the main components (measured via HPLC or fluorescence, see Supplementary Figs. 1 and 3).
