Fig. 2: TfpB is a phylogenetically distinct PilB homolog that is required for efficient T4P extension in A. baylyi. | Nature Communications

Fig. 2: TfpB is a phylogenetically distinct PilB homolog that is required for efficient T4P extension in A. baylyi.

From: Acinetobacter baylyi regulates type IV pilus synthesis by employing two extension motors and a motor protein inhibitor

Fig. 2

a Natural transformation assays of indicated strains. Each data point represents a biological replicate (n = 4) and bar graphs indicate the mean ± SD. LD limit of detection. ComP is the major pilin in A. baylyi, and the mutant is used here as a negative control. b Percent of piliated cells in pilT mutant populations of indicated strains. Each data point represents an independent, biological replicate (n = 3) and bar graphs indicate the mean ± SD. For each biological replicate, a minimum of 70 total cells were assessed. ND no pili detected. c Representative images of indicated strains labeled with AF488-mal with background fluorescence subtracted. Scale bars, 2 µm. d A rooted phylogeny of TfpB homologues found among Gammaproteobacteria. Branches are colored according to protein annotations in IMG, and nodes with bootstrap values greater than or equal to 70% are indicated by black circles. Black stars are at the tips of branches representing A. baylyi proteins with indicated protein names. Source data are provided as a Source Data file.

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