Fig. 3: Natural transformation is regulated by the inhibitor protein, CpiA, whose expression is controlled by the transcriptional repressor, CpiR. | Nature Communications

Fig. 3: Natural transformation is regulated by the inhibitor protein, CpiA, whose expression is controlled by the transcriptional repressor, CpiR.

From: Acinetobacter baylyi regulates type IV pilus synthesis by employing two extension motors and a motor protein inhibitor

Fig. 3

a Schematic of cpiRA locus organization. Numbers in parentheses are ACIAD numbers associated with each gene. b Natural transformation assays of the indicated strains performed with or without 100 µM IPTG added as indicated. Each data point represents a biological replicate (n = 4) and bar graphs indicate the mean ± SD. Pink bars denote a strain where cpiA is expressed at an ectopic location under the control of an IPTG-inducible promoter. c Fluorescence intensity of V. cholerae strains harboring a PcpiA-GFP reporter and a PBAD-cpiR ectopic expression construct as indicated when grown with or without 0.2% arabinose as indicated. Each data point represents a biological replicate (n = 2) and bar graphs indicate the mean ± SD. d Western blot showing CpiA-FLAG production in the indicated strains and PilB levels when CpiA is expressed. RpoA was detected as a loading control. Source data are provided as a Source Data file.

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