Fig. 3: scRNA-seq differentiation trajectory of in vitro derived Leydig cells. | Nature Communications

Fig. 3: scRNA-seq differentiation trajectory of in vitro derived Leydig cells.

From: TCF21+ mesenchymal cells contribute to testis somatic cell development, homeostasis, and regeneration in mice

Fig. 3

A Single-cell RNA-seq time course analysis of in vitro Leydig differentiation (days 0, 4, 7, and 14) identifies seven clusters, as visualized in t-SNE space. Note: n = 1 replicate per timepoint, but in vitro differentiation was successfully completed 8 times in prior experiments. B Heatmap of differentially expressed markers across the seven cluster centroids. C Visualization of the contribution of each individual time point at day 0, 4, 7, or 14 to the 7 clusters in t-SNE space. D Pseudotime ordering of cells from the 4 time points (days 0, 4, 7, and 14) by Monocle3 in UMAP space, colored by 7 clusters. E Schematic annotation of differentiation trajectory as defined by Monocle. F Expression profiles of selected markers across the differentiation pseudotime.

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