Fig. 4: BRD4 recruits MED1 and p65 to form SEs.

a The knockdown of BRD4 impaired both p65 and MED1 recruitments to SEs in TP63 (n = 3 per group). b The knockdown of BRD4 impaired both p65 and MED1 recruitments to SEs in MET (n = 3 per group). c JQ1 treatment or p65 knockdown significantly inhibited the recruitment of both CDK9 and CCNT1 to SEs in TP63 (n = 3 per group). d JQ1 treatment or p65 knockdown significantly inhibited the recruitment of both CDK9 and CCNT1 to SEs in MET (n = 3 per group). e RT-qPCR showed that knockdown of p65 and MED1 significantly inhibited the expression of TP63, MET, BIRC3, and MMP3 (n = 3 per group). f Increased enrichments of both p65 and BRD4 on SEs of TP63 in CSC-like cell (n = 3 per group). g Increased enrichments of both p65 and BRD4 on SEs of MET in CSC-like cells (n = 3 per group). h CSCs had significantly higher TP63 and MET expression levels (n = 3 per group). Data are presented as mean values ± SD in a–m. Statistical analysis was performed using two-tailed unpaired Student’s t-test. The data in a–m are representative of three experiments with similar results. Source data are provided as a Source data file.