Fig. 2: A subpopulation of muscle cells is not derived from the Pax3 lineage in mouse limbs.
From: Unexpected contribution of fibroblasts to muscle lineage as a mechanism for limb muscle patterning
a Transverse forelimb sections of E15.5 Pax3Cre:R26stop/Tom mice immunostained with Tomato (Pax3 lineage, red) antibody and DAPI staining (blue). b Focus on a muscle immunostained with Tomato (Pax3 lineage, red) and PAX7 (green) antibodies. c High magnifications showing PAX7+ cells (green) that are tomato-negative, i.e. not of Pax3 lineage (arrows). d Percentage of PAX7+/Tomato− cells versus PAX7+ cells in muscles. Data are presented as mean values ± SD from 3 limbs. e Longitudinal sections along a limb muscle of E15.5 Pax3Cre:R26H2BGFP mice immunostained with GFP (Pax3 lineage, green) and PAX7 (red) antibodies. Arrows point to PAX7+ cells that are GFP-negative. f Percentage of PAX7+/GFP− cells versus PAX7+ cells in muscles. Data are presented as mean values ± SD from 3 limbs. g Longitudinal sections along a limb muscle of E15.5 Pax3Cre:R26H2BGFP mice immunostained with GFP (Pax3 lineage, green) and MYOD (red) antibodies. Arrows point to MYOD+ cells that are GFP-negative. h Percentage of MYOD+/GFP− cells versus MYOD+ cells in E15.5 muscles. Data are presented as mean values ± SD from 3 limbs.
