Fig. 3: EED-deficient TEC affect thymocyte development at early and late stages.

a Distribution of B cells and T lineage precursors within DN cells. b Quantification of ETP, DN2, DN3 and preDP subsets within DN T cell precursors. c Analysis of maturational progression from CD8 ISP to CD71^pos DP thymocyte stages. Characterization of the developmental progression within CD8 (d) and CD4 lineage (e) selected thymocytes. f Quantification of re-circulated peripheral T cells within mature CD24^neg SP subsets. Representative flow cytometry contour plots are shown, bar graphs display mean values with SD together with individual data points from n = 4 Eed^fl/fl and n = 3 Eed^fl/fl::β5tCre mice (a–c) or n = 6 Eed^fl/fl and n = 9 Eed^fl/fl::β5tCre mice (d–f) at 3 weeks of age. ^*p < .05, ^**p < .01, ^***p < .001 (two-tailed unpaired Student’s t test). Data in bar graphs are from one experiment representative of three independent experiments. Eed^fl/fl::β5tCre: white bars with grey diamonds, Eed^fl/fl grey bars with black circles. n = biologically independent replicates per group. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 (two-tailed unpaired Student’s t test). The gating for thymocyte subsets is displayed in Supplementary Fig. 3. Source data including exact statistical test values are provided in the Source Data file.