Fig. 6: Peripheral T cells and their function in Eedfl/fl::β5tCre and Eedfl/fl mice. | Nature Communications

Fig. 6: Peripheral T cells and their function in Eedfl/fl::β5tCre and Eedfl/fl mice.

From: Indispensable epigenetic control of thymic epithelial cell development and function by polycomb repressive complex 2

Fig. 6

a Absolute numbers of splenic B and T cells in Eedfl/fl (n = 3) and Eedfl/fl::β5tCre mice (n = 3) at 4 weeks of age. b Representative contour plots and frequencies of lymph-node-derived T cell subsets. Frequency of TCRβ+ T cells, the CD4/CD8 ratio within TCRβ+ T cells and percentage of Foxp3+ TReg in CD4 T cells. c Frequencies of activated cells (CD44+CD62L−) in Foxp3+ TReg and frequencies of naive (CD44−CD62L+), central memory (CD44+CD62L+) and effector memory (CD44+CD62L−) T cells within the populations of Foxp3− CD4 and CD8 lymph node cells. b, c Eedfl/fl (n = 3) and Eed:fl/fl:β5tCre mice (n = 4) at 4 weeks of age. d Proliferative response and IL-2 secretion of naive lymph node CD4 T cells (CD44loCD25−) from Eedfl/fl (n = 3) and Eedfl/fl::β5tCre mice (n = 3) at 4 weeks of age. Representative histograms of CFSE dilution (including model components used for expansion index calculations; grey dotted lines) following stimulation for three days with CD3 mAb (1 µg/ml) and irradiated Rag2−/− splenocytes. Bar graphs display the mean expansion index (left) and mean IL2 concentrations in the supernatant (right) with SD. e Representative contour plots and frequencies of lymph-node-derived TReg subsets defined by their differential expression of ICOS and CD103 from Eedfl/fl (n = 3) and Eedfl/fl::β5tCre mice (n = 4) at 4 weeks of age. f T cell suppression assay: CD3 mAb-induced proliferation of sorted naive WT CD4 T cells (CD44loCD25−) in the presence of sorted lymph node Treg (CD4+CD25+) from Eedfl/fl (n = 3) and from pools of two Eedfl/fl::β5tCre mice each (n = 3) of mixed sex at 7–9 weeks of age. *p < 0.05, **p < 0.01, ***p < 0.001 (unpaired Student’s t test). Data in graphs show mean ± SD and are representative of two independent experiments. Contour plots (b, c, e) and histograms (d) are representative of data in bar graphs. Eedfl/fl::β5tCre: white bars with grey diamonds, Eedfl/fl grey bars with black circles. n = biologically independent replicates per group. Source data including exact statistical test values are provided in the Source Data file.

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