Fig. 9: Single-cell annotation of mTEC lineages. | Nature Communications

Fig. 9: Single-cell annotation of mTEC lineages.

From: Indispensable epigenetic control of thymic epithelial cell development and function by polycomb repressive complex 2

Fig. 9

a A UMAP plot showing all datasets projected together. mTEC cell type annotation was inferred from the combination of the two reference datasets. b The same UMAP projection showing only single-cell RNA-seq data on mTECs from this study. Eedfl/fl (magenta), Eedfl/fl::ZsGreen::β5tCre: ZsGreen-positive (green) and ZsGreen-negative (blue). c Volcano plots showing enrichment of TEC subtypes for (left panel) Eedfl/fl::ZsGreen::β5tCre ZsGreen+ vs. Eedfl/fl mTEChi, (middle panel) Eed:fl/fl:ZsGreen::β5tCre ZsGreen+ vs. Eedfl/fl::ZsGreen::β5tCre ZsGreen− mTEChi and (right panel) Eedfl/fl::ZsGreen::β5tCre ZsGreen− vs. Eedfl/fl mTEChi. All TEC subtypes showing nominally significant differences are labelled. Violin plots showing transcription factor network expression for transcription factor networks upregulated in (d) Eed:fl/fl:ZsGreen::β5tCre ZsGreen+ mTEChi, and (e) Eedfl/fl::ZsGreen::β5tCre ZsGreen− mTEChi. Eedfl/fl (magenta), Eedfl/fl::ZsGreen::β5tCre: ZsGreen-positive (green) and ZsGreen-negative (blue). Source data including exact statistical test values are provided in the Source Data file.

Back to article page