Fig. 5: Overexpression of NOTCH1 intracellular domain in small cell lung cancer induces a high neuroendocrine to low neuroendocrine transition marked by upregulation of antigen processing and presentation genes.
From: Notch signaling and efficacy of PD-1/PD-L1 blockade in relapsed small cell lung cancer
a Experimental design to assess neuroendocrine (NE) and antigen processing and presentation (APM) markers in suspension and adherent cells after NOTCH1 intracellular domain (N1ICD) overexpression. b Brightfield microscopic images of NCI-H82 suspension and adherent cells. Red arrows point to examples of adherent cells. c Immunoblot analysis of Notch pathway, NE and immune proteins after N1ICD activation for 15 days. Expression of downstream d Notch and YAP pathway, e NE and f APM transcripts. Bars indicate mean of three technical replicates. The data are presented as fold change compared with non-doxycycline treated cells. Three biological replicates at 16, 19, and 27 days after N1ICD overexpression are shown. Source data are provided as a Source Data file.
