Fig. 5: Identification of TFs binding to BG4-IP regions in 93T449 cells.

A Consensus sequences of TFBSs that are significantly enriched in BG4 ChIP peaks, as calculated by Homer software. AP-1: p value 1e−490, SP1: p value 1e−172. B Position and frequency of TFBSs with respect to the BG4 ChIP peak center. Data points for each TFBS occurrence are reported and fitted according to a nonparametric spline regression curve, the gray area surrounding the curve represents the confidence interval as measure of the regression likelihood. C Percentage of genes with (yellow) and without (darkblue) ChIP-G4s, oG4s, and pG4s containing validated TFBS for AP-1 and SP1, according to ENCODE database. D Western blot showing co-immunoprecipitation of G4s and the two TFs SP1 and AP-1. The INPUT lane 1 corresponds to the total fraction of the sheared chromatin used as starting material. G4s were immunoprecipitated by BG4 antibody (IP BG4), and AP-1 and SP1 were detected by immunoblotting (lane 2). AP-1 (lanes 4 and 5) and SP1 (lanes 6 and 7) TFs were immunoprecipitated from the sheared chromatin with or without previous incubation in the presence of BG4 antibody. Mock G (lane 3) and A (lane 8) are the negative controls immunoprecipitated in the absence of primary antibody using protein-G- or protein-A-coated beads, respectively. The shown blots belong to one of at least two independent biological replicates performed for each sample. Source data for each panel are provided or referenced in the Source data file.