Table 1 Stool protein markers that best distinguish UC from HC, or CD from HC, based on ELISA validation assays.

From: Predicting disease course in ulcerative colitis using stool proteins identified through an aptamer-based screen

Protein1

Healthy

U. Colitis

Crohn’s

Ulcerative colitis versus healthy control

Crohn’s disease versus healthy control

Mean (median)

Mean (median)

Mean (median)

Fold Change2

Cut-off3

AUC

Sensitivity

Specificity

Fold change2

Cut-off3

AUC

Sensitivity

Specificity

Adiponectinn

738 (19)

8403 (102)

5088 (55)

11.4

3146

0.62(0.38–0.84)

0.40(0.12–0.74)

0.92(0.73–0.99)

6.9

871

0.57(0.43–0.70)

0.33(0.19–0.50)

0.88(0.67–0.97)

Calprotectinp

2355 (651)

12540 (12,261)

5036 (672)

5.3

12118

0.7(0.47–0.98)

0.70(0.34–0.93)

0.96(0.79–0.99)

2.1***

9898

0.59(0.44–0.73)

0.31(0.17–0.47)

0.96(0.78–0.99)

Ferritinn

291 (245)

658 (335)

951 (532)

2.3*

312

0.60(0.36–0.85)

0.70(0.35–0.93)

0.63(0.41–0.81)

3.3***

646

0.72(0.59–0.84)***

0.49(0.32–0.65)

0.96(0.78–0.99)

Fibrinogenn

131 (30)

119 (84)

75 (65)

0.9**

64

0.62(0.38–0.85)

0.70(0.34–0.93)

0.71(0.49–0.87)

0.6*

38

0.60(0.45–0.76)

0.69(0.52–0.83)

0.63(0.40–0.81)

LG3BPn

5120 (2490)

4601 (3722)

4204 (2238)

0.9

1225

0.53(0.32–0.73)

0.90(0.56–0.99)

0.42(0.22–0.63)

0.8

1155

0.51(0.35–0.66)

0.77(0.60–0.88)

0.42(0.22–0.63)

Haptoglobinµ

5 (0)

300 (246)

119 (6)

61.4****

13

0.91(0.77–1.05)****

0.90(0.55–0.99)

0.92(0.73–0.99)

24.3**

3

0.71(0.59–0.83)**

0.56(0.39–0.72)

0.92(0.73–0.99)

Hemoglobinm

57 (1)

237 (292)

166 (214)

4.2***

276

0.87(0.70–1.02)****

0.70(0.35–0.93)

1.00(0.85–1.00)

2.9****

21

0.79(0.68–0.91)****

0.82(0.66–0.92)

0.67(0.44–0.84)

Lipocalin-2n

58 (53)

147 (102)

87 (75)

2.5**

88

0.70(0.45–o.094)

0.70(0.35–0.93)

0.75(0.53–0.90)

1.5*

63

0.63(0.49–0.77)

0.67(0.49–0.81)

0.63(0.41–0.81)

Lysozymeµ

10 (11)

11 (11)

10 (12)

1.1

8

0.55(0.35–0.76)

1.00(0.85–1.00)

0.33(0.16–0.55)

1.1

9

0.52(0.36–0.68)

0.82(0.66–0.92)

0.38(0.18–0.59)

MMP-12n

2 (0)

1215 (301)

85 (10)

763.4****

111

0.98(0.93–1.02)****

0.90(0.55–0.99)

1.00(0.85–1.00)

53.4***

18

0.74(0.64–0.85)****

0.44(0.28–0.60)

1.00(0.85–1.00)

MMP-8n

539 (684)

4821 (886)

991 (822)

8.9

1048

0.62(0.39–0.86)

0.40(0.12–0.74)

0.92(0.73–0.99)

1.8

1003

0.62(0.48–0.75)

0.44(0.28–0.60)

0.92(0.73–0.99)

MMP-9n

59 (68)

984 (140)

129 (99)

16.7**

161

0.70(0.47–0.93)

0.50(0.12–0.81)

1.00(0.85–1.00)

2.2*

162

0.68(0.55–0.82)**

0.36(0.21–0.52)

1.00(0.85–1.00)

Myeloperoxidasen

1264 (361)

21846 (20,293)

21657 (15675)

17.3****

3301

0.97(0.93–1.01)****

1.00(0.85–1.00)

0.91(0.72–0.99)

17.1****

2739

0.90(0.84–0.97)****

0.74(0.58–0.87)

0.91(0.72–0.99)

PGRP-Sn

143 (84)

3687 (1417)

1537 (513)

25.8****

172

0.96(0.91–1.01)****

1.00(0.85–1.00)

0.88(0.67–0.97)

10.7****

126

0.88(0.80–0.97)****

0.87(0.73–0.96)

0.83(0.62–0.95)

Properdinn

0 (0)

3422 (1212)

625 (0)

3421629.6****

193

0.89(0.74–1.03)****

0.80(0.44–0.97)

1.00(0.85–1.00)

625347.4*

44

0.55(0.46–0.64)

0.21(0.09–0.36)

1.00(0.85–1.00)

Proteinase-3µ

92 (30)

680 (177)

1024 (83)

7.4

336

0.68(0.46–0.90)

0.50(0.19–0.81)

0.96(0.78–0.99)

11.1*

73

0.69(0.55–0.82)**

0.54(0.37–0.69)

0.96(0.79–0.99)

Resistinn

2 (0)

290 (108)

139 (6)

162.4**

212

0.75(0.55–0.96)*

0.50(0.18–0.81)

1.00(0.85–1.00)

77.9**

6

0.72(0.60–0.84)**

0.51(0.35–0.68)

0.96(0.79–0.99)

Serpin A4n

26 (28)

98 (81)

92 (57)

3.7*

81

0.73(0.50–0.96)*

0.56(0.21–0.86)

0.96(0.79–0.99)

3.5**

57

0.72(0.59–0.84)***

0.51(0.35–0.67)

0.92(0.73–0.99)

TIMP-1p

1 (0)

156 (122)

42 (11)

145.6****

20

1.00(1.00–1.00)****

1.00(0.85–1.00)

1.00(0.85–1.00)

39.3**

9

0.74(0.63–0.85)****

0.54(0.37–0.69)

1.00(0.85–1.00)

TIMP-2p

0 (0)

25 (6)

0 (0)

3494.2****

0.44

0.95(0.85–1.05)****

0.90(0.55–0.99)

1.00(0.85–1.00)

66.6

0.37

0.58(0.51–0.66)*

0.21(0.09–0.36)

1.00(0.85–1.00)

  1. 1Biomarker proteins are listed bolded for clarity; n = ng/ml, p = pg/ml, µ = ug/ml, m = mg/ml.
  2. 2Fold change reflects disease vs HC. Shown p-values are determined using the Mann–Whitney U test (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).
  3. 3Cut-off values for AUC were determined using the Youden index.
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