Fig. 7: BS-DRL1 KO mice show progressive Purkinje cell degeneration. | Nature Communications

Fig. 7: BS-DRL1 KO mice show progressive Purkinje cell degeneration.

From: Long noncoding RNA BS-DRL1 modulates the DNA damage response and genome stability by interacting with HMGB1 in neurons

Fig. 7

a, b Representative images showing immunostaining of Purkinje cells (in green) and quantification of Purkinje cells. Immunofluorescence staining was performed with brain sections prepared from 3-month-old naive mice and calbindin antibody. Scale bar: 50 μm. Data are presented as mean ± SD. For each group, n = 3. ns not significant. c, d Same as (a, b) except the brain sections were prepared from 12-month-old naive mice. Scale bar: 50 μm. Data are presented as mean ± SD. For each group, n = 3. ****p < 0.0001. e, f Representative images of immunostaining and quantification for NeuN-positive neurons in M1 cortex of naive 12-month-old mice. Scale bar:100 μm. Data are presented as mean ± SD. For each group, n = 3. **p < 0.01. g, h TUNEL staining and quantification of neurons transduced with indicated shRNA virus and treated with vehicle or ETO for 1 h. Four images per group and 28–34 cells per image were used for statistic. Scale bar:10 μm. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ns not significant. n = 3 biologically independent samples.

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