Fig. 1: βFaar has lower-than-normal expression in islets of obese mouse models.

a Total islet RNA harvested from db/db mice (n = 5) and wide type mice (n = 7) was screened by RNA-sequence analysis. Microarray heatmap of differentially expressed lncRNAs. b qRT-PCR was performed to test the βFAAR (V1–V4) expression in the human islets. c The expression levels of βFaar in different tissues of C57BL/6J mice compared to islet (n = 5). White adipose tissue (WAT), brown adipose tissue (BAT). d The expression levels of βFaar in the islets at different stages (after 0-week, 4-week, 6-week, 8-week, and 16-week feeding HFD) during the development of obesity inducing diabetes (n = 5). The expression levels of βFaar in HFD mice (e, n = 5) and in db/db mice (f, n = 5). g The expression levels of βFaar in the pancreas, islets and exocrine glands (n = 3). Pancreas was digested by collagenase VI, all islet were picked out, other cells were exocrine glands. h βFaar was enriched in the MIN6 cell and primary islet cell cytoplasm fraction. i FISH analysis of βFaar in MIN6 cells and islet cells. The nuclei were stained with DAPI. Magnification: ×40 or ×20, scale bar, 10 μm or 20 μm. All experiments above were performed in triplicates, and each group contained three batches of individual samples. The p-values by two-tailed unpaired Student’s t test (e, f), one-way ANOVA (c, d, g) are indicated. Data represent the mean ± SD. Source data are provided as a Source data file.