Fig. 3: Suppression of βFaar expression decreases insulin transcription and secretion.
From: The long non-coding RNA βFaar regulates islet β-cell function and survival during obesity in mice
βFaar overexpression plasmid (oe-βFaar) and βFaar smart silence (si-βFaar) were transfected into mice islet cells for 48 h. Then mRNA levels of insulin genes (Ins1 and Ins2, a, n = 3) were tested by qRT-PCR, insulin content (b, n = 5) and insulin secretion was analyzed by ELISA (c, n = 5). INSULIN gene expression (d), insulin content (e) and insulin secretion (f) in the human islets transfected with oe-βFAAR or sg-βFAAR. Insulin secretion in the primary islets of db/db mice transfected with oe-βFaar or si-βFaar (g, n = 5, 10 weeks old). The mRNA (h) and protein (i) level of CREB1 and NEUROD1. j IPGTT (2 g kg-1) in overnight fasted Len-βFaar mice and control mice (n = 9). k In vivo insulin excursions in overnight fasted Len-βFaar mice and control mice after IPGTT exposure (n = 9). l Relative Ins1 and Ins2 expression levels in vivo (n = 3). m Static insulin secretion of islets (n = 5) at indicated glucose concentrations. n–o The protein levels of CREB1 and NEUROD1 in the islets of Len-βFaar mice (n) or βFaar-KO mice (o) (n = 5). All experiments above were performed in triplicates, and each group contained three batches of individual samples. The p-values by one-way ANOVA (b, d, e), or two-way ANOVA (a, c, f–h), and (j–m) are indicated. Data represent the mean ± SD, except (j, k) (mean ± SEM). Source data are provided as a Source data file.
